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By Gerard Aihaud

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Extra resources for Adipose Tissue Protocols (Methods in Molecular Biology Vol 155)

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133, 143–164. 72. , and Ricquier, D. (1993) Beta 3-adrenoceptor agonist ICI-D7114 is not as efficient on reinduction of uncoupling protein mRNA in sheep as it is in dogs and smaller species. J. Anim. Sci. 71, 2388–2394. 73. Loncar, D. (1991) Convertible adipose tissue in mice. Cell Tissue Res. 266, 149–161. 74. , Mayers, R. , Briscoe, M. , and Holloway, B. R. (1991) Beta 3-adrenergic receptor stimulation restores message and expression of brown-fat mitochondrial uncoupling protein in adult dogs.

R. (1994) Physiological review: regional adiposity and morbidity. Am. Physiol. Soc. 74, 761–798. Choosing an AT Depot 17 48. Abate, N. and Carg, A. (1995) Heterogeneity in adipose tissue metabolism: causes, implications and management of regional adiposity. Prog. Lipid Res. 34, 53–70. 49. Marette, A. , et al. (1997) Regional variations in adipose tissue insulin action and GLUT4 glucose transporter expression in severely obese premenopausal women. Diabetologia 40, 590–598. 50. , and Després, J. P.

Given that sections are 3–4 µm in thickness, that the thickness of brown adipocytes is 30–50 µm, and that of white adipocytes is 80–150 µm, it is theoretically possible to perform different reactions on the same cell in different sections. 4. Incubation (see Note 1) Our immunologic sequence is indirect. The specific Ab (primary antibody), raised against the Ag to be localized, binds directly to the tissue Ag in the section. Another Ab against the primary Ab (called “bridge” serum), conjugated to one biotin molecule, represents the second step of the sequence.

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