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Extra resources for Adipose Tissue Protocols (Methods in Molecular Biology Vol 155)
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Given that sections are 3–4 µm in thickness, that the thickness of brown adipocytes is 30–50 µm, and that of white adipocytes is 80–150 µm, it is theoretically possible to perform different reactions on the same cell in different sections. 4. Incubation (see Note 1) Our immunologic sequence is indirect. The specific Ab (primary antibody), raised against the Ag to be localized, binds directly to the tissue Ag in the section. Another Ab against the primary Ab (called “bridge” serum), conjugated to one biotin molecule, represents the second step of the sequence.