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Extra resources for Advances in lipid research. Volume 2
Cereus have been investigated by Kates et al. (1962). , 1944). The unsaponifiable material consists largely of long chain alcohols (mainly n-C16 and n-C18) with traces of long chain aldehydes. Since only traces of free alcohols are present in the intact lipids, the alcohols probably occur in the cell in an esterified form. The phosphatides consist chiefly of phosphatidylethanolamine (36%), phosphatidylglycerol (30%), and polyglycerol phosphatide (10%). Several minor components were identified tentatively, on the basis of staining behavior and Rf value, as lecithin ( 8 % ) , lysophosphatidylethanolamine (10%), and lysolecithin ( < 1 % ) ; an unidentified ninhydrin-positive component ( 5 % ) was also present.
Marcescens had high proportions of unsaturated acids and low values for cyclopropane acids, whereas pigmented cultures had high proportions of cyclopropane acids and low proportions of unsaturated acids. When the changes in fatty acid composition were followed with time, during the growth of pigmented and unpigmented cells, both cultures showed the same kind of changes observed by Law et al. (1963), the rate of formation of the cyclopropane acids being somewhat slower relative to cell growth for the unpigmented culture.
1964). The lipids of M. halodenitrificans, a moderate halophilic micrococcus (requiring 1 M salt for optimum growth) have been studied by Kates et al. (1961). , 1955), some glyceride material, and component(s) yielding unsaponifiable material. The phosphatides consist chiefly of phosphatidylethanolamine and phosphatidylglycerol, with smaller amounts of unidentified acidic phosphatides ( polyglycerol phosphatides and phosphatidylinositol? ), and a ninhydrin-positive phosphatide, tentatively identified as lysophosphatidylethanolamine.